New spectrophotometric and chemometric methods were carried out for the simultaneous assay of trelagliptin (TRG) and its acid\ndegradation product (TAD) and applied successfully as a stability indicating assay to recently approved ZafatekÃ?® tablets. TAD was\nmonitored using TLC to ensure complete degradation. Furthermore, HPLC was used to confirm dealing with one major acid\ndegradation product. The proposed methods were developed by manipulating zero-order, first-derivative, and ratio spectra of\nTRG and TAD using simultaneous equation, first-derivative, and mean-centering methods, respectively. Using Spectra Manager\nII and Minitab v.14 software, the absorbance at 274 nmââ?¬â??260.4 nm, amplitudes at 260.4 nmââ?¬â??274.0 nm, and mean-centered values at\n287.6 nmââ?¬â??257.2nm were measured against methanol as a blank for TRG and TAD, respectively. Linearity and the other validation\nparameters were acceptable at concentration ranges of 5ââ?¬â??50 Ã?¼g/mL and 2.5ââ?¬â??25 Ã?¼g/mL for TRG and TAD, respectively. Using oneway\nanalysis of variance (ANOVA), the optimized methods were compared and proved to be accurate for the simultaneous assay\nof TRG and TAD.
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